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Related Experiment Videos

Formulation and process parameters affecting protein encapsulation into PLGA microspheres during ethyl acetate-based

M Cho1, H Sah

  • 1Catholic University of Daegu, Hayang-Up, Gyeongsan City, Gyeongbuk, South Korea.

Journal of Microencapsulation
|July 16, 2005
PubMed
Summary
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Controlling ethyl acetate quenching time is key for efficient protein encapsulation into poly(lactic-co-glycolic acid) (PLGA) microspheres. Optimizing this process improves lysozyme encapsulation efficiency and microsphere characteristics.

Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Polymer Chemistry

Background:

  • Protein encapsulation in microspheres is crucial for controlled drug release.
  • Poly(lactic-co-glycolic acid) (PLGA) microspheres are widely used for drug delivery.
  • Optimizing the microencapsulation process is essential for achieving high encapsulation efficiency.

Purpose of the Study:

  • To investigate formulation and process parameters affecting protein encapsulation into PLGA microspheres.
  • To understand the impact of ethyl acetate quenching on lysozyme encapsulation efficiency.
  • To optimize the double emulsion microencapsulation process for better protein loading.

Main Methods:

  • Utilized a water-in-oil-in-water double emulsion method for protein encapsulation.

Related Experiment Videos

  • Emulsified lysozyme solution in ethyl acetate with PLGA 75:25.
  • Varied ethyl acetate quenching times (5, 15, 30, 45 min) to study its effect.
  • Quantified lysozyme using bicinchoninic acid assay.

    • Main Results:

    • Lysozyme leaching occurred during primary emulsion stirring, with efficiencies ranging from 4.79% to 51.9%.
    • Ethyl acetate quenching prevented droplet fragmentation and induced PLGA precipitation, forming microspheres.
    • Encapsulation efficiency varied significantly with quenching time, from 9.89% to 75.82%.

    Conclusions:

    • The timing of ethyl acetate quenching critically influences protein encapsulation efficiency and microsphere size.
    • Optimization of quenching onset and formulation parameters can lead to desirable protein encapsulation efficiencies.
    • This study provides insights for improving PLGA microsphere formulation for protein delivery.