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Related Experiment Videos

Poly(D,L-lactide-co-glycolide) protein-loaded nanoparticles prepared by the double emulsion method--processing and

U Bilati1, E Allémann, E Doelker

  • 1University of Geneva, Switzerland.

Journal of Microencapsulation
|July 16, 2005
PubMed
Summary

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Optimizing nanoparticle formulation for drug delivery is crucial. Sonication during double emulsion improves protein drug entrapment efficiency, while polymer properties and solvent choice significantly impact loading and particle size.

Area of Science:

  • Biomaterials Science
  • Pharmaceutical Technology
  • Nanotechnology

Background:

  • Extensive research exists on biodegradable microparticles for peptide/protein drugs.
  • However, critical parameters affecting drug entrapment in nano-scaled carriers remain under-investigated.

Purpose of the Study:

  • To investigate formulation and processing parameters influencing nanoparticle size and drug loading using the w(1)/o/w(2) double emulsion method.
  • To evaluate the impact of emulsification techniques, polymer properties, and solvent choice on drug entrapment efficiency and particle characteristics.

Main Methods:

  • Utilized the w(1)/o/w(2) double emulsion method for nanoparticle preparation.
  • Employed fluorescein isothiocyanate-labelled bovine serum albumin (FITC-BSA) as a model protein drug.

Related Experiment Videos

  • Compared sonication versus vortex mixing for emulsification steps.
  • Investigated effects of polymer properties (molecular weight, hydrophilicity, end groups), drug loading, inner phase volume, organic solvent, and polymer concentration.

    • Main Results:

    • Sonication for both emulsification steps achieved high FITC-BSA entrapment efficiencies (>80%), independent of mixing duration/intensity.
    • Vortex mixing for the primary emulsion resulted in poor entrapment (~25%).
    • Polymer properties like high molecular weight, hydrophilicity, and free carboxylic end groups enhanced entrapment.
    • Low drug loading, large inner aqueous phase volume, and methylene chloride as solvent favored high entrapment (up to 100%).
    • Methylene chloride increased mean particle size due to larger particles; increased polymer concentration also raised mean particle size.

    Conclusions:

    • Sonication is a key processing parameter for achieving high drug entrapment efficiency in nano-scaled double emulsions.
    • Polymer characteristics and formulation parameters significantly influence drug loading and particle size.
    • Careful selection of solvent and formulation conditions is necessary to balance high entrapment efficiency with desired particle characteristics for drug delivery applications.