Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glucuronidation of bioflavonoids by human UGT1A10: structure-function relationships.

R H Lewinsky1, P A Smith, P I Mackenzie

  • 1Department of Clinical Pharmacology, Flinders University School of Medicine, Flinders Medical Centre, Bedford Park, SA, Australia.

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
|July 16, 2005
PubMed
Summary

The human UDP glucuronosyltransferase 1A10 (UGT1A10) enzyme metabolizes dietary bioflavonoids. Structural features like hydroxyl groups on the A-ring are key for UGT1A10 to act as a substrate.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Influence of Migration Timing and Local Conditions on Reproductive Timing in Arctic-Breeding Birds.

Ecology and evolution·2025
Same author

Hydrogen Bonding in Amorphous Indomethacin.

Pharmaceutics·2024
Same author

Set up to 'fail'? Implementing contamination minimisation procedures and environmental monitoring in a Sexual Assault Referral Centre.

Journal of forensic and legal medicine·2022
Same author

The visualisation of fingermarks on Pangolin scales using gelatine lifters.

Forensic science international·2020
Same author

Disrupting wildlife crime: The benefits of meaningful collaboration.

Forensic science international·2019
Same author

Plastic and Non-plastic Debris Ingestion in Three Gull Species Feeding in an Urban Landfill Environment.

Archives of environmental contamination and toxicology·2017

Area of Science:

  • Pharmacology
  • Biochemistry
  • Drug Metabolism

Background:

  • Extrahepatic human UDP glucuronosyltransferase 1A10 (UGT1A10) is present in the gastrointestinal tract.
  • UGT1A10 is involved in eliminating orally ingested lipophilic compounds.
  • The substrate specificity of UGT1A10 for these compounds, particularly bioflavonoids, is not fully understood.

Purpose of the Study:

  • To characterize the structural features of bioflavonoids that enable them to be substrates for UGT1A10.
  • To understand the role of UGT1A10 in the metabolism of dietary bioflavonoids.

Main Methods:

  • Investigated the glucuronidation of various bioflavonoids by UGT1A10.
  • Analyzed the impact of hydroxyl group positions and other substituents on the A-ring and B-ring of bioflavonoids on UGT1A10 activity.

Related Experiment Videos

Main Results:

  • UGT1A10 prefers substrates with hydroxyl groups at C6 or C7 of the A-ring, but not at C5.
  • Glucuronidation is hindered by hydroxyl groups on the B-ring (C4') and by sugar moieties.
  • Increased activity was observed with up to two additional hydroxyl groups on the A-ring.

Conclusions:

  • Specific hydroxyl group placement on the A-ring is crucial for bioflavonoid glucuronidation by UGT1A10.
  • UGT1A10 significantly contributes to the gastrointestinal metabolism of dietary bioflavonoids.
  • Understanding these interactions is vital for predicting the metabolic fate of dietary compounds.