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Updated: Aug 1, 2026

RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells
Published on: August 25, 2010
Genome-wide RNAi screen for host factors required for intracellular bacterial infection
Hervé Agaisse1, Laura S Burrack, Jennifer A Philips
1Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA. herve.agaisse@yale.edu
Abstract:
Most studies of host-pathogen interactions have focused on pathogen-specific virulence determinants. Here, we report a genome-wide RNA interference screen to identify host factors required for intracellular bacterial pathogenesis. Using Drosophila cells and the cytosolic pathogen Listeria monocytogenes, we identified 305 double-stranded RNAs targeting a wide range of cellular functions that altered L. monocytogenes infection. Comparison to a similar screen with Mycobacterium fortuitum, a vacuolar pathogen, identified host factors that may play a general role in intracellular pathogenesis and factors that specifically affect access to the cytosol by L. monocytogenes.
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