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Related Experiment Video

Updated: Jul 12, 2026

Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model

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Published on: July 29, 2012

The Trypanosoma cruzi proteome.

J A Atwood¹, D B Weatherly, T A Minning

¹Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.

Science (New York, N.Y.)

|July 16, 2005

Summary

This study analyzed Trypanosoma cruzi proteomes across its life cycle, identifying over 2,700 proteins and novel mucin-associated surface proteins. Distinct energy sources, like histidine and fatty acids, fuel different parasite stages.

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Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs

Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs

Published on: June 23, 2022

Area of Science:

Proteomics
Parasitology
Genomics

Background:

Trypanosoma cruzi is a kinetoplastid parasite responsible for Chagas disease.
Understanding T. cruzi's life cycle and molecular mechanisms is crucial for developing interventions.

Purpose of the Study:

To perform a comprehensive proteomic analysis of all four life-cycle stages of Trypanosoma cruzi.
To complement existing genomic data with proteomic insights.
To identify novel proteins and understand metabolic adaptations.

Main Methods:

Whole-organism proteomic analysis.
Peptide identification and mapping against the annotated T. cruzi genome.
Comparative analysis across four distinct parasite life-cycle stages.

Main Results:

Identified peptides mapping to 2,784 proteins within 1,168 protein groups.
Characterized over 1,000 "hypothetical" proteins, including a new mucin-associated surface protein family.
Revealed distinct energy source utilization: histidine in insect stages and fatty acids in intracellular amastigotes.

Conclusions:

The study provides a detailed proteomic landscape of T. cruzi across its life cycle.
Identified novel protein families and stage-specific metabolic strategies.
This proteomic data is vital for understanding T. cruzi biology and for future drug target discovery.