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Related Experiment Video

Updated: Jul 20, 2026

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

TILLING moves beyond functional genomics into crop improvement.

Ann J Slade¹, Vic C Knauf

¹Anawah Inc., 1102 Columbia Street, Suite 600, Seattle, WA 98104, USA.

Transgenic Research

|July 19, 2005

Summary

Targeting Induced Local Lesions IN Genomes (TILLING) offers a non-transgenic approach for crop improvement. This method aids in gene discovery and trait modulation, overcoming limitations of transgenic techniques in many important crop species.

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Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

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Area of Science:

Plant genetics
Functional genomics
Crop improvement

Background:

Transgenic methods are widely used for crop trait improvement but are impractical for reverse genetics in many commercial crops.
This limits gene function studies and the development of novel cultivars.
A non-transgenic reverse genetics approach is needed to overcome these limitations.

Purpose of the Study:

To introduce and demonstrate the utility of Targeting Induced Local Lesions IN Genomes (TILLING) as a non-transgenic method for reverse genetics.
To highlight TILLING's potential for functional genomics and trait modulation in crop plants.

Main Methods:

Development and application of the TILLING method.
Induction and identification of novel genetic variation.
Demonstration in the model plant Arabidopsis thaliana and extension to crop plants.

Main Results:

TILLING has been successfully developed and demonstrated as a viable non-transgenic reverse genetics approach.
The method effectively induces and identifies useful genetic variation.
TILLING shows promise for application in diverse crop species.

Conclusions:

Targeting Induced Local Lesions IN Genomes (TILLING) provides a powerful non-transgenic alternative for reverse genetics in crops.
This method facilitates gene function discovery and trait improvement in a wide range of crop plants.
TILLING is a versatile tool for both functional genomics and crop breeding.