Related Experiment Video

Updated: Aug 17, 2026

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
10:42

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

Transcriptional responses of human epidermal keratinocytes to Oncostatin-M

Nika Finelt1, Alix Gazel, Steven Gorelick

  • 1Department of Dermatology, NYU School of Medicine, 550 First Avenue, New York, NY 10016, United States.

Cytokine
|July 19, 2005
PubMed

Insights

Oncostatin-M (OsM) significantly alters gene expression in skin keratinocytes, impacting immunity and cell growth. These molecular responses occur in distinct early and late phases following treatment.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Immunology

Background:

  • Oncostatin-M (OsM) is implicated in skin inflammation and oncogenesis, such as psoriasis and Kaposi sarcoma.
  • The precise molecular mechanisms of OsM action in keratinocytes remain incompletely understood.

Purpose of the Study:

  • To comprehensively analyze the transcriptional landscape of primary human epidermal keratinocytes in response to OsM.
  • To identify key genes and pathways regulated by OsM in skin cells.

Main Methods:

  • Transcriptional profiling using high-density DNA microarrays.
  • Treatment of primary human epidermal keratinocytes with OsM.
  • Analysis of gene expression at multiple time points (1h and 48h).

Main Results:

  • OsM treatment profoundly impacts gene expression in keratinocytes.
  • Key affected areas include innate immunity, angiogenesis, cell adhesion, motility, tissue remodeling, cell cycle, and transcription.
  • Gene expression changes occur in two distinct waves: early (1h) and late (48h).
  • Primary targets of OsM regulation include secreted factors, receptors, and nuclear transcription factors, which mediate secondary effects.

Conclusions:

  • OsM is a potent regulator of keratinocyte function with broad molecular effects.
  • The identified gene expression patterns provide insights into OsM's role in skin inflammatory and oncogenic processes.
  • Understanding these responses can inform therapeutic strategies for OsM-related skin conditions.

More Related Videos

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis07:29

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Published on: May 16, 2020

Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...
Cells of the Epidermis01:24

Cells of the Epidermis

The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
Layers of the Epidermis01:21

Layers of the Epidermis

The epidermis, the outermost layer of the skin, is composed of several distinct layers. From deep to superficial, the layers of the epidermis are as follows:
Stratum Basale
Stratum basale, also known as the stratum germinativum, is the deepest layer of the epidermis. It is composed of a single layer of actively dividing cells called basal cells or basal keratinocytes. These cells constantly undergo cell division to replenish the upper layers of the epidermis. Additionally, melanocytes, which...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.