Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exploring Mycobacterium avium inhibition by macrocyclic compounds.

Suzana David1, Vanessa Barros, Krassimira Passos Guerra

  • 1Mycobacteriology Unit, Instituto de Higiéne e Medicina Tropical/Universidade Nova de Lisboa, Rua da Junqueira 96, 1349-008 Lisbon, Portugal. suzanadavid@IHMT.UNL.PT

Research in Microbiology
|July 19, 2005
PubMed
Summary

Synthetic macrocyclic compounds (MCCs) show promise against Mycobacterium avium. N-acetate derivatives like TETA demonstrated significant in vitro and intracellular inhibition, suggesting potential for new anti-M. avium therapies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations between Cues of Sexual Desire and Sexual Attitudes in Portuguese Women.

European journal of investigation in health, psychology and education·2021
Same author

Complexation of <i>C</i>-Functionalized Cyclams with Copper(II) and Zinc(II): Similarities and Changes When Compared to Parent Cyclam Analogues.

Inorganic chemistry·2021
Same author

Corrigendum: Ecophysiological Traits of Invasive C<sub>3</sub> Species <i>Calotropis procera</i> to Maintain High Photosynthetic Performance Under High VPD and Low Soil Water Balance in Semi-Arid and Seacoast Zones.

Frontiers in plant science·2020
Same author

Zinc(ii) and copper(ii) complexes as tools to monitor/inhibit protein phosphorylation events.

Dalton transactions (Cambridge, England : 2003)·2020
Same author

Ecophysiological Traits of Invasive C<sub>3</sub> Species <i>Calotropis procera</i> to Maintain High Photosynthetic Performance Under High VPD and Low Soil Water Balance in Semi-Arid and Seacoast Zones.

Frontiers in plant science·2020
Same author

Monitoring inorganic pyrophosphatase activity with the fluorescent dizinc(ii) complex of a macrocycle bearing one dansylamidoethyl antenna.

Dalton transactions (Cambridge, England : 2003)·2020

Area of Science:

  • Medicinal Chemistry
  • Microbiology
  • Drug Discovery

Background:

  • Mycobacterium avium infections pose a significant health challenge.
  • Synthetic macrocyclic compounds (MCCs) are being explored for antimicrobial properties.
  • Tetraazamacrocycles with various pendant arms represent a class of MCCs with potential therapeutic applications.

Purpose of the Study:

  • To evaluate the in vitro activity and intracellular clearance of synthetic macrocyclic compounds against Mycobacterium avium.
  • To investigate the structure-activity relationships of different N-pendant arms on macrocyclic compounds.
  • To assess the potential of these compounds as novel therapeutic agents for M. avium infections.

Main Methods:

  • In vitro antimicrobial assays against Mycobacterium avium laboratory strains.

Related Experiment Videos

  • Intracellular clearance studies using the murine macrophage cell line J-774.
  • Evaluation of iron(III) complexes of the macrocyclic compounds.

    • Main Results:

    • Several synthetic macrocyclic compounds, particularly those with N-acetate pendant arms (e.g., TETA), exhibited significant in vitro inhibition of M. avium.
    • Inhibition levels of over 90% were achieved at 50 mg/l for the most active compounds.
    • Intracellular activity of TETA confirmed its in vitro efficacy, while iron complexation influenced inhibitory effects for some compounds.

    Conclusions:

    • Synthetic macrocyclic compounds, especially N-acetate derivatives, show significant potential for developing new treatments against Mycobacterium avium.
    • The pendant arm structure and potential metal chelation play crucial roles in the observed antimicrobial activity.
    • Further investigation into MCCs is warranted for their development as a new class of anti-M. avium drugs.