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Mechanical loading-induced gene expression and BMD changes are different in two inbred mouse strains.

Chandrasekhar Kesavan1, Subburaman Mohan, Susanna Oberholtzer

  • 1Musculoskeletal Disease Center, Veterans Affairs Loma Linda Healthcare System, Loma Linda, CA 92357, USA.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|July 19, 2005
PubMed
Summary

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Mechanical loading enhances bone formation in mice, with C57B1/6J mice showing a greater anabolic response than C3H/HeJ mice due to increased bone formation and decreased bone resorption marker gene expression.

Area of Science:

  • Skeletal biology
  • Mechanobiology
  • Genetics

Background:

  • Mechanical loading is crucial for bone health.
  • Understanding age- and strain-dependent responses is key to developing bone therapies.

Purpose of the Study:

  • To evaluate the skeletal anabolic response to mechanical loading in different age groups and mouse strains (C57B1/6J and C3H/HeJ).
  • To analyze changes in bone size, bone mineral density (BMD), and gene expression.

Main Methods:

  • Applied mechanical loads (6-9 N) to the tibia of mice using a four-point bending model.
  • Measured bone parameters via peripheral quantitative computed tomography (pQCT).
  • Assessed gene expression of bone formation (BF) and bone resorption (BR) markers using real-time PCR.

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Main Results:

  • C57B1/6J mice showed increased BMD with higher loads, unlike C3H/HeJ mice.
  • Significant increases in periosteal circumference and total bone area were observed in both strains.
  • Mechanical loading modulated BF and BR marker gene expression, with greater changes in C57B1/6J mice.

Conclusions:

  • Mechanical loading activates molecular pathways for bone anabolism.
  • C57B1/6J mice exhibit a more pronounced anabolic response to mechanical loading compared to C3H/HeJ mice.
  • Age did not significantly alter the bone's response to bending in the studied groups.