Gene expression changes induced by a recombinant E1-/E3- adenovirus type 5 vector in human mammary epithelial cells
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Published on: June 7, 2019
Angelo G Scibetta1, John Copier, Angela Barrett
1Cancer Research UK, Breast Cancer Biology Group, Guys Hospital, London, UK. angelo.scibetta@cancer.org.uk
Objectives:
Adenoviral vectors are used in transferring exogenous genes to a variety of cells and tissue types both in vitro and in vivo. Gene expression changes induced by an E1/E3-defective adenovirus vector have been studied in human mammary epithelial cells by comparing the gene expression profile in infected and uninfected cells.
Methods:
The human mammary epithelial cell line HB2 was infected with an E1/E3-defective adenovirus type 5 vector. Total RNA was extracted from infected and uninfected cells 24 and 72 h after infection and subjected to microarray analysis using the Affymetrix U133A genomic chip system. Semiquantitative RT-PCR confirmed the regulation of genes observed by microarray analysis.
Results:
The microarray analysis showed 24 and 95 transcripts to be regulated 24 and 72 h after infection, respectively. A relatively high number of genes involved in innate and inflammatory host immune responses, including interleukin-8, interleukin-6, NF-kappaB(2), RELB and fos, were induced. As expected from an E1-defective virus, changes in the expression of genes involved in the G1-S transition and in the activation of cell proliferation were not detected.
Conclusion:
Our study provides insight into the host transcriptional response following transduction of an adenoviral vector into mammary epithelial cells.
