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Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
09:08

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

Variations in the nucleocapsid protein gene of infectious bronchitis viruses isolated in Korea

Ji Yeun Park1, Son Il Pak, Haan Woo Sung

  • 1Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Kangwon National University, Chunchon.

Virus Genes
|July 19, 2005
PubMed

Insights

This study analyzed infectious bronchitis viruses (IBVs) in Korea, revealing distinct genetic clusters and potential recombination events among strains. Understanding IBV evolution is key for poultry health.

Area of Science:

  • Veterinary Virology
  • Molecular Epidemiology
  • Poultry Disease Research

Background:

  • Infectious Bronchitis Virus (IBV) poses a significant threat to the global poultry industry.
  • Characterizing circulating IBV strains is crucial for effective disease control and vaccine development.
  • Previous studies have highlighted the genetic diversity of IBV, but specific data from Korea requires detailed investigation.

Purpose of the Study:

  • To genetically characterize infectious bronchitis virus (IBV) isolates from Korea between 2001 and 2003.
  • To compare the nucleocapsid (N) protein gene sequences of Korean IBV strains with non-Korean isolates.
  • To elucidate the phylogenetic relationships and potential origins of Korean IBV strains.

Main Methods:

  • Isolation of fourteen infectious bronchitis viruses (IBVs) from infected chickens in Korea.
  • Amplification of nucleocapsid (N) protein genes using reverse transcriptase-polymerase chain reaction (RT-PCR).
  • Nucleotide and amino acid sequence analysis, including phylogenetic comparisons with global IBV strains.

Main Results:

  • Korean IBV isolates exhibited significant amino acid sequence similarity among themselves (89.2%–98.3%) and with non-Korean strains (57.0%–96.6%).
  • Phylogenetic analysis segregated Korean IBV isolates into three distinct clusters, with some differing from S1 glycoprotein analysis.
  • One cluster comprised exclusively Korean IBV isolates, while others included international strains, suggesting recombination events.

Conclusions:

  • The genetic diversity of IBV in Korea is substantial, with evidence of distinct evolutionary pathways.
  • Phylogenetic analysis of the N protein gene provides valuable insights into IBV strain relationships and origins.
  • Recombination between IBV strains of different origins may contribute to the emergence of novel viral variants in Korea.

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