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Updated: Aug 16, 2026

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
Genomic analysis of Grapevine Retrotransposon 1 (Gret 1) in Vitis vinifera
H Sofia Pereira1, Augusta Barão, Margarida Delgado
1Secção de Genética, Instituto Superior de Agronomia, Centro de Botânica Aplicada à Agricultura, Tapada da Ajuda, 1349-017 Lisboa, Portugal. sofiapereira@isa.utl.pt
Abstract:
The complete sequence of the first retrotransposon isolated in Vitis vinifera, Gret 1, was used to design primers that permitted its analysis in the genome of grapevine cultivars. This retroelement was found to be dispersed throughout the genome with sites of repeated insertions. Fluorescent in situ hybridization indicated multiple Gret 1 loci distributed throughout euchromatic portions of chromosomes. REMAP and IRAP proved to be useful as molecular markers in grapevine. Both of these techniques showed polymorphisms between cultivars but not between clones of the same cultivar, indicating differences in Gret 1 distribution between cultivars. The combined cytological and molecular results suggest that Gret 1 may have a role in gene regulation and in explaining the enormous phenotypic variability that exists between cultivars.
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