Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Laser-guided direct writing for three-dimensional tissue engineering.

Yaakov Nahmias1, Robert E Schwartz, Catherine M Verfaillie

  • 1Department of Biomedical Engineering, University of Minnesota, Minneapolis, 55455, USA.

Biotechnology and Bioengineering
|July 19, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FLIM quality metric visualization as a means to validate consistency across large-area non-homogeneous FLIM datasets.

Methods and applications in fluorescence·2026
Same author

Mechanistic modeling predicts efficacy of CISH knockout in tumor-infiltrating lymphocytes with synergistic gene editing.

Physical biology·2026
Same author

Engineering "physically optimized" T cells for increased sampling of complex tumor microenvironments.

bioRxiv : the preprint server for biology·2026
Same author

Biophysical modeling identifies an optimal hybrid amoeboid-mesenchymal mechanism for maximal T cell migration speeds.

Cell reports·2026
Same author

Importance of De Novo Gene Evolution to Emerging Viral Threats: The ORF10 Strain-Restricted Orphan Gene of SARS-CoV-2 Promotes Pathogenesis.

Molecular biology and evolution·2025
Same author

Increasing <i>SOX10</i> Expression Overcomes Schizophrenia-Associated Early Oligodendrocyte Growth Deficits In Vitro.

Biological psychiatry global open science·2025
Same journal

Polycationic Peptide Engineering of Phage Endolysins Expands Host Range and Enhances Antibacterial and Antibiofilm Activities Against Bacillus Species.

Biotechnology and bioengineering·2026
Same journal

Artificial Intelligence-Powered Algal Biodiesel: The Future of Biofuel Production Through Data-Driven Biotechnology.

Biotechnology and bioengineering·2026
Same journal

Developing Anti-EGFR/Anti-HER2 Bifunctional Antibody for Solid Tumors by Protein Engineering.

Biotechnology and bioengineering·2026
Same journal

Bridging Organ-on-a-Chip and Omics: A Multi-Dimensional Frontier in Biomedical Research.

Biotechnology and bioengineering·2026
Same journal

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice.

Biotechnology and bioengineering·2026
Same journal

Characterization and Therapeutic Potential of a Novel Lytic Phage-Derived Endolysin PA16cLys Against Uropathogenic Pseudomonas aeruginosa Biofilms.

Biotechnology and bioengineering·2026
See all related articles

Laser-guided direct writing (LGDW) precisely patterns human umbilical vein endothelial cells (HUVEC) to create vascular structures in engineered tissues. This technique enables the reconstruction of native tissue architecture for improved nutrient transport and cell survival.

Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Cell Biology

Background:

  • Engineered tissue size is limited by oxygen and nutrient transport due to the lack of vascularization.
  • Rapid cell death occurs in engineered tissues within hours without a vascular bed.
  • Vascularization of implanted tissues by blood vessels can take days, hindering immediate function.

Purpose of the Study:

  • To investigate the use of laser-guided direct writing (LGDW) for creating vascular structures in engineered tissues.
  • To demonstrate the ability of LGDW to pattern endothelial cells with micrometer accuracy in 2D and 3D.
  • To assess the self-assembly of patterned endothelial cells into functional vascular networks.

Main Methods:

  • Utilized laser-guided direct writing (LGDW) to pattern human umbilical vein endothelial cells (HUVEC).

Related Experiment Videos

  • Patterned HUVEC on Matrigel in both two- and three-dimensional formats.
  • Co-cultured patterned HUVEC vascular structures with hepatocytes to form hepatic sinusoid-like structures.

    • Main Results:

    • LGDW achieved micrometer-accurate patterning of HUVEC in 2D and 3D.
    • Patterned HUVEC self-assembled into vascular structures following the desired LGDW pattern.
    • Co-culturing with hepatocytes resulted in aggregated tubular structures resembling hepatic sinusoids.

    Conclusions:

    • LGDW is a viable technique for fabricating vascular networks within engineered tissues.
    • This method allows for precise control over tissue architecture at the cellular level.
    • The capability facilitates studies on tissue morphogenesis, cell interactions, and angiogenesis.