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SMRT has tissue-specific isoform profiles that include a form containing one CoRNR box.
Stephen Short1, Marianne Malartre, Colin Sharpe
1University of Portsmouth, School of Biological Sciences and Institute of Biomolecular and Biomedical Sciences, King Henry Building, King Henry I St, Portsmouth PO1 2DY, UK.
Biochemical and Biophysical Research Communications
|July 20, 2005
Summary
The nuclear receptor corepressor SMRT (Silencers of Retinoid and Thyroid hormone Receptors) exhibits alternative splicing, generating isoforms with varying CoRNR boxes. This allows for tissue-specific regulation of transcription factors.
Area of Science:
- Molecular Biology
- Gene Regulation
- Biochemistry
Background:
- SMRT (Silencers of Retinoid and Thyroid hormone Receptors) functions as a corepressor for numerous transcription factors.
- The SMRT protein contains distinct domains: N-terminal for repression and C-terminal for nuclear receptor interaction via CoRNR boxes.
Purpose of the Study:
- To investigate the alternative splicing of SMRT transcript and its impact on isoform generation.
- To understand the tissue-specific expression and regulation of SMRT isoforms in mice and frogs.
Main Methods:
- Analysis of SMRT primary transcript and its alternative splicing patterns.
- Examination of SMRT isoform profiles in different mouse tissues and frog tissues.
Main Results:
- Alternative splicing of the SMRT transcript leads to variations in CoRNR box inclusion.
- A novel, abundant SMRT isoform with a single CoRNR box was identified in mice.
- Mouse tissues express SMRT isoforms with one, two, or three CoRNR boxes.
- Frog SMRT isoform profiles are tissue-specific, and mice exhibit differential expression of SMRT transcript isoforms.
Conclusions:
- The generation of multiple SMRT isoforms through alternative splicing provides a mechanism for fine-tuning transcriptional regulation.
- Tissue-specific expression and regulation of SMRT isoforms allow cells to control the specific transcription factors modulated by SMRT.