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Related Experiment Videos

Development of a multiplexed microbioreactor system for high-throughput bioprocessing.

Nicolas Szita1, Paolo Boccazzi, Zhiyu Zhang

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., 66-566 Cambridge, MA 02139, USA.

Lab on a Chip
|July 20, 2005
PubMed
Summary

This study introduces a multiplexed microbioreactor system for parallel microbial fermentation. The system enables real-time monitoring of optical density, dissolved oxygen, and pH, demonstrating reproducible results comparable to larger bioreactors.

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Microbial Fermentation Technology

Background:

  • Traditional microbial fermentation often requires large-scale equipment and lacks parallel processing capabilities.
  • Real-time monitoring of key fermentation parameters is crucial for process optimization and reproducibility.

Purpose of the Study:

  • To develop and validate a multiplexed microbioreactor system for parallel microbial fermentation.
  • To enable in-situ, real-time monitoring of optical density (OD), dissolved oxygen (DO), and pH.
  • To assess the system's reproducibility and its utility in comparing different reactor designs.

Main Methods:

  • Fabrication of microbioreactors (150 microl working volume) using poly(methylmethacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS).

Related Experiment Videos

  • Integration of miniature magnetic stirrers and optical sensors for real-time OD, DO, and pH monitoring.
  • Parallel fermentation experiments using Escherichia coli in four stirred microbioreactors.

    • Main Results:

    • Demonstrated reproducible performance of the multiplexed microbioreactor system during parallel Escherichia coli fermentations.
    • Achieved favorable comparisons of OD, DO, and pH profiles with traditional bench-scale bioreactor systems.
    • Successfully utilized the system to quantify differences between two distinct microbioreactor designs.

    Conclusions:

    • The developed multiplexed microbioreactor system offers a robust platform for parallel microbial fermentation.
    • The system provides accurate, real-time monitoring and demonstrates high reproducibility.
    • It is suitable for comparative studies of reactor designs and process optimization.