Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diffusion based analysis in a sheath flow microchannel: the sheath flow T-sensor.

Matthew S Munson1, Kenneth R Hawkins, Melissa S Hasenbank

  • 1University of Washington, Department of Bioengineering, Box 352255, Seattle, WA, USA. msmunson@u.washington.edu

Lab on a Chip
|July 20, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Heat-actuated valve implementation in a point-of-care, paper-based microfluidic device for infectious disease detection.

PloS one·2026
Same author

A low-cost point-of-care device for the simultaneous detection of two sexually transmitted bacterial pathogens in vaginal swab samples.

The Analyst·2025
Same author

High-sensitivity detection of <i>Mycobacterium tuberculosis</i> DNA in tongue swab samples.

Journal of clinical microbiology·2025
Same author

Detection of Mycobacterium tuberculosis from tongue swabs using sonication and sequence-specific hybridization capture.

PloS one·2024
Same author

High-sensitivity detection of <i>Mycobacterium tuberculosis</i> DNA in tongue swab samples.

medRxiv : the preprint server for health sciences·2024
Same author

UbiNAAT: a multiplexed point-of-care nucleic acid diagnostic platform for rapid at-home pathogen detection.

Lab on a chip·2024

This study presents a novel microfluidic channel for analyzing adsorbing species via diffusion. The sheath flow T-sensor accurately measured diffusion coefficients for molecules and proteins without surface passivation.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Biophysics

Background:

  • Diffusion-based analysis is crucial for understanding molecular interactions.
  • Microfluidic devices offer precise control over fluid dynamics.
  • Surface passivation is often required to prevent adsorption, complicating analyses.

Purpose of the Study:

  • To develop a microfluidic channel for diffusion analysis without surface passivation.
  • To validate the utility of a sheath flow T-sensor for measuring diffusion coefficients.
  • To demonstrate the method's applicability to both small molecules and proteins.

Main Methods:

  • Utilized a microfluidic channel with a sheath flow configuration.
  • Analyzed the spatial distribution of fluorescently labeled species.

Related Experiment Videos

  • Calculated diffusion coefficients from the derivative of the intensity profile at the fluid interface.

    • Main Results:

    • Successfully measured the diffusion coefficient of rhodamine B (small molecule).
    • Successfully measured the diffusion coefficient of wheat germ agglutinin (protein).
    • Demonstrated that the sheath flow T-sensor functions effectively without surface passivation.

    Conclusions:

    • The developed microfluidic channel and sheath flow T-sensor enable diffusion-based analysis of adsorbing species.
    • The method is versatile, applicable to molecules of varying sizes.
    • Eliminates the need for surface passivation, simplifying experimental procedures.