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Protective antibody responses elicited by a meningococcal outer membrane vesicle vaccine with overexpressed

Victor C Hou1, Oliver Koeberling, Jo Anne Welsch

  • 1Children's Hospital Oakland Research Institute, Oakland, California 94609, USA.

The Journal of Infectious Diseases
|July 20, 2005
PubMed

Insights

A novel meningococcal outer membrane vesicle (OMV) vaccine, engineered to overexpress genome-derived neisserial antigen (GNA) 1870, provides broader protection against meningococcal disease. This GNA1870-OMV vaccine reduces the risk of PorA-escape mutants compared to conventional OMV vaccines.

Area of Science:

  • Vaccinology
  • Microbiology
  • Immunology

Background:

  • Meningococcal outer membrane vesicle (OMV) vaccines are effective but target specific PorA proteins, risking immune escape.
  • Neisseria meningitidis harbors potential vaccine targets like genome-derived neisserial antigen (GNA) 1870.

Purpose of the Study:

  • To evaluate a novel OMV vaccine engineered to overexpress GNA 1870.
  • To assess the breadth of immune response and potential for immune selection compared to existing vaccines.

Main Methods:

  • Engineered a Neisseria meningitidis strain to overexpress GNA 1870 for OMV vaccine preparation.
  • Immunized mice with the modified GNA1870-OMV vaccine, recombinant GNA1870, or wild-type OMV.
  • Assessed serum bactericidal antibody responses, complement deposition, and passive protection in infant rats.

Main Results:

  • The GNA1870-OMV vaccine elicited broader serum bactericidal antibody responses than control vaccines.
  • Antiserum showed increased C3 complement deposition on N. meningitidis and enhanced passive protection.
  • N. meningitidis with reduced PorA expression was more susceptible to anti-GNA1870 antibodies.

Conclusions:

  • The modified GNA1870-OMV vaccine offers broader protection against meningococcal disease.
  • This vaccine candidate presents a lower risk of selecting PorA-escape mutants compared to conventional OMV vaccines.

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