Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Laser-assisted microdissection of membrane-mounted tissue sections.

Lise Mette Gjerdrum1, Stephen Hamilton-Dutoit

  • 1Institute of Pathology, Aarhus University Hospital, Denmark

Methods in Molecular Biology (Clifton, N.J.)
|July 21, 2005
PubMed
Summary

Laser-assisted microdissection (LAM) isolates specific cells from complex biological tissues. This technique improves molecular analysis accuracy by ensuring the cellular origin of detected molecules is known.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alpha 2A adrenergic receptor antagonism reduces fibrosis, inflammation and portal hypertension in models of chronic liver disease.

JHEP reports : innovation in hepatology·2026
Same author

Epidemiological evidence for the role of puberty and immune senescence in Hodgkin lymphoma aetiology from 1992 Danish cases.

International journal of cancer·2025
Same author

Circulating tumour DNA as a prognostic tool for surgically treated pancreatic ductal adenocarcinoma.

Human pathology·2025
Same author

Sinonasal neuroendocrine carcinoma in a previously healthy young adult male.

BMJ case reports·2025
Same author

Drug Interactions With Tamoxifen and Treatment Effectiveness in Premenopausal Breast Cancer Patients: A Bayesian Joint Modeling Approach.

Pharmacoepidemiology and drug safety·2025
Same author

Genetic variants in tamoxifen metabolism and early treatment discontinuation among premenopausal breast cancer patients.

Breast cancer research and treatment·2025

Area of Science:

  • Histology
  • Molecular Biology
  • Biotechnology

Background:

  • Biological tissues are complex mixtures of cells and matrices.
  • Tissue heterogeneity complicates molecular analysis of whole extracts.
  • Identifying the cellular origin of molecules is crucial for accurate interpretation.

Purpose of the Study:

  • To describe laser-assisted microdissection (LAM) for isolating specific cell populations.
  • To detail protocols for ultraviolet laser-based tissue microdissection.
  • To enable accurate molecular analysis of heterogeneous biological specimens.

Main Methods:

  • Laser-assisted microdissection (LAM) using an ultraviolet laser system.
  • Isolation of specific target cell populations or tissue components.

Related Experiment Videos

  • Sample preparation for subsequent molecular analyses, including polymerase chain reaction.

    • Main Results:

    • LAM provides enriched samples of specific cell types.
    • Enables identification of target-specific molecular alterations in heterogeneous tissues.
    • Facilitates more accurate detection and quantification of target molecules.

    Conclusions:

    • LAM is a leading histological technique for targeted molecular studies.
    • Ultraviolet laser microdissection enhances the precision of molecular analysis.
    • This method is essential for studying molecular changes in diseased tissues.