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CDK5 regulates cell-cell and cell-matrix adhesion in human keratinocytes

N Nakano1, A Nakao, K Ishidoh

  • 1Atopy (Allergy) Research Center, Juntendo University School of Medicine, Tokyo, Japan. nbnakano@med.juntendo.ac.jp

The British Journal of Dermatology
|July 21, 2005
PubMed
Abstract

Insights

Cyclin-dependent kinase 5 (CDK5) and its activator p35 play roles in skin cell adhesion. Reduced CDK5/p35 expression in psoriasis may contribute to the disease, impacting cell-matrix interactions.

Area of Science:

  • Cell Biology
  • Dermatology
  • Biochemistry

Background:

  • Cyclin-dependent kinase 5 (CDK5) is a serine/threonine kinase activated by non-cyclin partners p35 and p39.
  • CDK5 is crucial for neuronal development, influencing adhesion, migration, and differentiation.

Purpose of the Study:

  • To investigate the expression and functions of CDK5 in human keratinocytes.
  • To explore the role of CDK5/p35 in cell-cell and cell-matrix adhesion in skin.

Main Methods:

  • Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblotting were used to study CDK5/p35 expression and interactions.
  • Aggregation/adhesion assays assessed roles in cell-cell and cell-matrix adhesion.
  • Immunohistochemistry examined CDK5/p35 localization in normal and psoriatic epidermis.

Main Results:

  • CDK5 and p35 were expressed in keratinocytes and normal human epidermis.
  • CDK5 inhibition affected cadherin-dependent cell aggregation and fibronectin adhesion via beta1 integrin.
  • Psoriatic skin showed reduced CDK5/p35 expression and decreased activated beta1 integrin.

Conclusions:

  • CDK5/p35 may regulate cadherins and integrins, influencing cell-cell and cell-matrix adhesion in keratinocytes.
  • Diminished CDK5/p35 expression in the epidermis might be implicated in psoriasis pathogenesis.

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