Short interval intradermal skin testing in farmed red deer (Cervus elaphus) inoculated with Mycobacterium bovis

Two groups of 26 red deer (Cervus elaphus) were tuberculin skin tested for 41 weeks at three and six week intervals respectively, except at 17 weeks when the internal was two and five weeks. Seventeen weeks after the start of the experiment 36 deer were inoculated intratracheally with Mycobacterium bovis, and the remaining 16 were run in-contact. At six weeks post inoculation, 35 of the 36 inoculated deer reacted to the skin test with a mean skin thickness difference (STD) of 6.3 mm. In inoculated deer further testing led to a suppression of skin sensitivity which was significantly greater in the three-weekly tested group. There was no statistical difference in skin thickness response between 1 and 2 mg/ml bovine purified protein derivative (PPD). Of 454 tests on noninoculated deer (noninfected), 107 produced reactions. These reactions were small and 96% had a STD of less than 2 mm.