Sustained strenuous exercise in sled dogs depresses three blood copper enzyme activities

Studies show mixed conclusions about acute responses of copper status to strenuous exercise. Because copper function involves metalloenzyme activities, which might take days to change, the present study examined the response of three copper metalloenzyme activities to sustained strenuous exercise in sled dogs. A race lasting 12-15 d depressed activities for both plasma ceruloplasmin and erythrocyte superoxide dismutase in dogs consuming commercial dog foods and meats. A shorter, 3-d training run for dogs fed a commercial balanced diet also depressed ceruloplasmin activities but not superoxide dismutase activities. Dogs fed the same diet but that did not run showed no changes in either parameter. Activities of a third copper enzyme, plasma diamine oxidase, also decreased after a 3-d training run. In summary, blood activities of three copper enzymes were depressed by sustained strenuous exercise in sled dogs.