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Immunohistochemical localization of advanced glycation end products in pinguecula
Yuichi Kaji1, Tetsuro Oshika, Shiro Amano
1Department of Ophthalmology, Tsukuba University Institute of Clinical Medicine, Tsukuba, Ibaraki 305-8565, Japan. sanken-tky@umin.ac.jp
Background:
Advanced glycation end products (AGEs) are known to be deposited in the target organ of ageing. In addition, the deposition of AGEs accelerate the process of ageing. We investigated the immunohistochemical localization of AGEs in pinguecula, one of the ocular changes related with ageing process.
Methods:
Surgical specimens of conjunctiva with or without pinguecula were prepared from nine patients, respectively. Immunohistochemical localization of AGEs was investigated using monoclonal antibodies to N(epsilon)-(carboxymethyl)lysine, pentosidine, imidazolone, and pyrraline.
Results:
Moderate to strong immunoreactivities to AGEs were detected in the subepithelial amorphous deposits of all the surgical specimens with pinguecula. In contrast, no or weak immunoreactivities to AGEs were detected in the surgical specimens without pinguecula.
Conclusions:
Pinguecula is an aggregation of AGEs-modified proteins. The presence of pinguecula would be an index of local irradiation of ultraviolet rays and decreased antioxidant activities.
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