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Updated: Aug 16, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
Published on: March 3, 2015
Cooperative evolution in protein complexes of yeast from comparative analyses of its interaction network
Massimo Vergassola1, Alessandro Vespignani, Bernard Dujon
1Unité Génomique des Microorganismes Pathogènes, Structure and Dynamics of Genomes Department, Institute Pasteur, CNRS URA 2171, 28 rue du Dr. Roux, F-757724 Paris Cedex 15, France. massimo@pasteur.fr
Abstract:
A comparative analysis among Saccharomyces cerevisiae and the other four yeasts Candida glabrata, Kluyveromyces lactis, Debaryomyces hansenii, and Yarrowia lipolytica is presented. The broad evolutionary range spanned by the organisms allows to quantitatively demonstrate novel evolutionary effects in protein complexes. The evolution rates within cliques of interlinked proteins are found to bear strong multipoint correlations, witnessing a cooperative coevolution of complex subunits. The coevolution is found to be largely independent of the tendency of the subunits to have similar abundances.
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