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Mycobacterium tuberculosis complex DNA does not exist in atheromatous plaques

Simin Rota1, S Tuncer, S Rota

  • 1Pamukkale University Medical School Department of Biochemistry, Denizli/Turkey. siminrota@yahoo.co.uk

The New Microbiologica
|July 23, 2005
PubMed

Insights

This study investigated Mycobacterium tuberculosis DNA in atherosclerotic plaques, finding none. Further research into mycobacteria

Area of Science:

  • Cardiovascular Research
  • Infectious Diseases
  • Molecular Biology

Background:

  • Infectious agents are implicated in atherosclerosis.
  • Mycobacterium tuberculosis (M. tuberculosis) and its Hsp65 antigen show links to atheroma formation in mice.
  • Antibody levels against Hsp65 are elevated in patients with atheromas.

Purpose of the Study:

  • To detect M. tuberculosis DNA in human atherosclerotic plaque samples using PCR.
  • To investigate the etiological role of M. tuberculosis in atherosclerosis.

Main Methods:

  • DNA extraction from endarterectomy tissue samples.
  • PCR amplification targeting the M. tuberculosis IS6110 region.
  • Electrophoretic analysis of PCR products.

Main Results:

  • M. tuberculosis DNA was not detected in any of the analyzed human atherosclerotic plaque samples.
  • The study did not find direct evidence of M. tuberculosis presence in these plaques.

Conclusions:

  • The direct detection of M. tuberculosis DNA in human atherosclerotic plaques was unsuccessful in this study.
  • Further etiological studies involving mycobacteriaceae are needed to elucidate their role in atherosclerosis pathogenesis.

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