Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Friction force microscopy as an alternative method to probe molecular interactions.

Małgorzata Lekka1, Andrzej J Kulik, Sylvia Jeney

  • 1The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland. malgorzata.lekka@ifj.edu.pl

The Journal of Chemical Physics
|July 23, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Low-Temperature Magnetotransport of Mixed Polycrystalline Rutile, Anatase and Brookite Phases of TiO<sub>2</sub>.

Materials (Basel, Switzerland)·2026
Same author

Multiscale structural-rheological mapping of cancer spheroids during maturation.

Soft matter·2026
Same author

Matrix Mechanics Governs Mechano-Metabolic Adaptation across Cancer Grades in Bladder Spheroids.

ACS applied materials & interfaces·2026
Same author

Chemokine-Receptor Modulation Shapes Neuroinflammatory and Biomechanical Responses in Organotypic Hippocampal Cultures After Oxygen-Glucose Deprivation.

ACS chemical neuroscience·2026
Same author

Indenting multicellular spheroids with various tip geometries.

European biophysics journal : EBJ·2026
Same author

Transient Spin Labeling of Plastics with Chlorine Dioxide.

ACS physical chemistry Au·2026

Friction force microscopy effectively distinguishes specific protein-carbohydrate binding from non-specific interactions. This technique offers a novel approach for studying molecular recognition in cellular processes.

Area of Science:

  • Biophysics
  • Surface Science
  • Molecular Biology

Background:

  • Protein-carbohydrate interactions are crucial for cellular recognition processes.
  • Lectins serve as molecular probes to investigate carbohydrate expression and structure.
  • Understanding these interactions is key to deciphering cellular communication.

Purpose of the Study:

  • To investigate protein-carbohydrate interactions using friction force microscopy (FFM).
  • To differentiate between specific molecular recognition and nonspecific interactions.
  • To assess the utility of FFM in studying cellular recognition events.

Main Methods:

  • Attaching lectins (concanavalin A and lentil lectin) to a probing tip.
  • Immobilizing carboxypeptidase Y on a modified glass surface via microcontact printing.

Related Experiment Videos

  • Analyzing friction force maps and loading rate dependencies in a physiological buffer.

    • Main Results:

    • FFM results were categorized into two distinct groups: molecular recognition and nonspecific interactions.
    • Specific interactions were observed for lectin-protein complexes.
    • Control measurements were attributed to nonspecific interactions, validating the method's specificity.

    Conclusions:

    • Friction force microscopy is a viable technique for studying molecular recognition events.
    • FFM provides insights into the specificity of protein-carbohydrate interactions.
    • This method holds promise for advancing the study of cellular recognition mechanisms.