Characterization of immobilized artificial membrane (IAM) and XTerra columns by means of chromatographic models
Elisabet Lázaro1, Clara Ràfols, Martí Rosés
1Department de Química Analítica, Universitat de Barcelona, Diagonal 647, E-08028 Barcelona, Spain.
Abstract:
Immobilized artificial membranes (IAMs) prepared from phosphatidylcholine analogs are used as stationary phases in liquid chromatography systems to model drug partitioning between an aqueous phase (mobile phase) and a cell membrane (IAM column). Two different chromatographic models, which describe retention as a function of solute and column-mobile phase properties, have been applied to characterization of an IAM and two reversed phase C18 columns (Waters XTerra MSC18 and XTerra RP18) with acetonitrile-water mobile phases. The comparison of the results shows that the phosphatidylcholine group makes IAM column more polar than both XTerra columns, specially in terms of hydrogen-bond acceptor ability. XTerra RP18 is slightly more polar than XTerra MSC18 because of the presence of the embedded carbamate polar group.
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