"In-gel patch electrophoresis:" a new method for environmental DNA purification
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Updated: Aug 16, 2026

Electroeluting DNA Fragments
Published on: September 5, 2010
Changhyun Roh1, Francois Villatte, Byung-Gee Kim
1School of Chemical Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, South Korea.
Abstract:
Most of the microorganism species are largely untapped and could represent an interesting reservoir of genes useful for biotechnological applications. Unfortunately, a major difficulty associated with the methods used to isolate environmental DNA is related to the contamination of the extracted material with humic substances. These polyphenolic compounds inhibit the DNA processing reactions and severely impede cloning procedures. In this work, we describe a rapid, simple, and efficient method for the purification of genomic DNA from environmental samples: we added a chromatography step directly embedded into an agarose gel electrophoresis. This strategy enabled the DNA extraction from various environmental samples and it appeared that the purity grade was compatible with digestion by restriction enzymes and polymerase chain reaction (PCR) amplifications.
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