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Improved CZE capabilities with new dynamic coatings.

William W P Chang1, David C Bomberger, Luke V Schneider

  • 1Target Discovery, Inc., 4015 Fabian Way, Palo Alto, CA 94303, USA. william_chang@targetdiscover.com

Journal of Capillary Electrophoresis and Microchip Technology
|July 27, 2005
PubMed
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Dynamic coatings called EOTrol allow independent control of electroosmotic flow (EOF) magnitude and direction in capillary electrophoresis (CE). This enables effective separation of challenging inorganic cations and zwitterions, overcoming pH-dependent limitations.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Electrochemistry

Background:

  • Electroosmotic flow (EOF) critically impacts resolution and separation times in capillary zone electrophoresis (CZE).
  • In bare-silica capillaries, EOF is pH and buffer-dependent, limiting separation design.
  • Traditional CZE methods struggle with separating species with small mobility differences or those requiring low pH conditions.

Purpose of the Study:

  • To introduce and evaluate EOTrol dynamic coatings for independent control of EOF.
  • To demonstrate the application of EOTrol in overcoming limitations of pH-dependent EOF in CZE.
  • To showcase EOTrol's utility in resolving challenging analyte mixtures.

Main Methods:

  • Utilized EOTrol dynamic coatings in capillary zone electrophoresis.

Related Experiment Videos

  • Applied EOTrol to separate inorganic cations with minimal mobility differences.
  • Employed EOTrol for rapid separation of organic zwitterions typically requiring low pH.

    • Main Results:

    • EOTrol coatings enabled adjustment of EOF magnitude and direction independently of pH and buffer composition.
    • Successful separation of inorganic cations with subtle mobility variations was achieved.
    • Rapid and effective separation of organic zwitterions was demonstrated using EOTrol.

    Conclusions:

    • EOTrol dynamic coatings offer a versatile solution for manipulating EOF in CZE.
    • Independent EOF control facilitates the design of more effective and efficient electrophoretic separations.
    • EOTrol broadens the applicability of CZE for complex analytical challenges.