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Updated: Aug 16, 2026

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

Immobilization of histidine-tagged recombinant proteins onto micropatterned surfaces for cell-based functional assays

Koichi Kato1, Hideki Sato, Hiroo Iwata

  • 1Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

Langmuir : the ACS Journal of Surfaces and Colloids
|July 27, 2005
PubMed

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Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...