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Updated: Aug 16, 2026

Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
Biocompatibility in vitro tests of mineral trioxide aggregate and regular and white Portland cements
Daniel Araki Ribeiro1, Marco Antonio Hungaro Duarte, Mariza Akemi Matsumoto
1Center for Genotoxins and Carcinogens Evaluation (TOXICAN), Department of Pathology, Botucatu Medical School, UNESP, SP Brazil. ak92@hotmail.com
Abstract:
Mineral trioxide aggregate (MTA) and Portland cement are being used in dentistry as root end-filling materials. However, biocompatibility data concerning genotoxicity and cytotoxicity are needed for complete risk assessment of these compounds. In the present study, genotoxic and cytotoxic effects of MTA and Portland cements were evaluated in vitro using the alkaline single cell gel (comet) assay and trypan blue exclusion test, respectively, on mouse lymphoma cells. The results demonstrated that the single cell gel (comet) assay failed to detect DNA damage after a treatment of cells by MTA and Portland cements for concentrations up to 1000 microg/ml. Similarly, results showed that none of the compounds tested were cytotoxic. Taken together, these results seem to indicate that MTA and Portland cements are not genotoxins and do not induce cellular death.
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