Synaptic organization of the serotoninergic input to the superficial gray layer of the hamster's superior colliculus

Immunocytochemistry with an antibody directed against a serotonin (5-HT)-bovine serum albumin (BSA) conjugate was combined with electron microscopy and serial section analysis to examine the synaptic organization of the serotoninergic projection to the stratum griseum superficiale (SGS) of the hamster's superior colliculus (SC). Most of the 5-HT-immunoreactive swellings in the hamster's SC did not make conventional synaptic contacts. Examination of 250 such swellings in single thin sections revealed only 11 synapses. Assessment of an additional 50 swellings followed through serial sections showed that only 6% (N = 3) of these varicosities made conventional synaptic contacts. All the synapses made by serotoninergic axons were with the dendrites of SC neurons. These results demonstrate that the inhibitory effects of 5-HT on SC neurons in hamster are mainly mediated by the nonsynaptic release of this indoleamine.