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Histologic assessment of mesh fixation following laser-assisted tissue soldering in a lapine model
Raymond J Lanzafame1, Philip Brondon, Istvan Stadler
1Rochester General Hospital Laser Center, 1445 Portland Avenue #202, Rochester, NY 14621-3095, USA. ray.lanzafame@viahealth.org
Objective:
Wound histology and mesh bioincorporation following intraperitoneal fixation using laser-assisted soldering was evaluated.
Methods:
2.8-3.2 kg NZW rabbits underwent laparotomy. Controls had 2x2 cm segments of Mersilene stapled to peritoneum. Group 2 segments were affixed with 55% collagen solder onlay by fiber-coupled diode laser (1.43 +/- 10 micro, 2.5 W CW, 4 mm spot, 60 degrees C set temperature). Group 4 had Mersilene inlaid into melted solder. Group 3 had solder-embedded Vicryl mesh affixed. Animals were euthanized at 0, 2, 4, 6 weeks. Fixed sections were assessed for integrity, inflammation, and fibrosis using H & E, Masson's Trichrome and Evans Van Gieson staining.
Results:
Histology demonstrated cell types, local mesh reaction, and progressive evidence of solder reabsorption mimicking normal healing and bioincorporation. Mersilene groups demonstrated normal arrangement of collagen-rich layers around mesh.
Conclusion:
Collagen-based tissue soldering permits normal wound healing and may mitigate use of staples. Further development of this strategy is warranted.
