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Quenched fluorescent substrate-based peptidase assays
Rebecca A Lew1, Nathalie Tochon-Danguy, Catherine A Hamilton
1Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|July 27, 2005
Abstract:
The use of specific quenched fluorescent substrates (QFS) provides a rapid and sensitive method to measure peptidase activity, and is readily adaptable to high-throughput screening of potential peptidase inhibitors. In this chapter, we discuss general considerations for the development of QFS assays, and describe in detail an assay protocol for the mammalian metallopeptidase, endothelin-converting enzyme.
