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[Mapping of apple Co gene using SSR markers]
Yi Ke Tian1, Cai Hong Wang, Hong Yi Dai
1The College of Life Science, Northwestern Sci-Tech University of Agriculture and Forestry, Yangling 712100.
Shi Yan Sheng Wu Xue Bao
|July 28, 2005
Summary
Researchers identified three SSR markers (COL, CH02a10, CH03d11) linked to the columnar apple (Co) gene. This advances understanding of apple tree form genetics and breeding potential.
Area of Science:
- Plant genetics
- Molecular biology
- Horticultural science
Context:
- Columnar apple trees are a valuable genetic resource for developing desirable tree forms in apple breeding.
- Understanding the genetic basis of columnar growth is crucial for efficient breeding programs.
- Previous research has identified the 'Co' gene as responsible for the columnar trait.
Purpose:
- To identify simple sequence repeat (SSR) markers linked to the columnar apple (Co) gene.
- To map the location of the Co gene on the apple genome using linkage analysis.
- To facilitate marker-assisted selection for columnar growth in apple breeding.
Summary:
- This study screened 106 F1 individuals from a 'Spur Fuji' x 'Telamon' cross using SSR markers.
- Bulked segregant analysis (BSA) identified three SSR markers (COL, CH02a10, CH03d11) linked to the Co gene on the tenth linkage group.
- Linkage analysis determined the genetic distances to the Co locus as 15.3 cM (COL), 22.2 cM (CH02a10), and 3.9 cM (CH03d11), positioning the Co gene between COL and CH03d11.
Impact:
- Provides tightly linked SSR markers for the Co gene, enabling marker-assisted selection in apple breeding.
- Contributes to the development of improved apple cultivars with desirable columnar growth habits.
- Enhances the understanding of apple genome organization and gene mapping for tree architecture traits.
