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Related Experiment Videos

Terminal complement complex in septic shock with capillary leakage: marker of complement activation?

T Schuerholz1, M Leuwer, M Cobas-Meyer

  • 1Friedrich Schiller University, Department of Anaesthesiology and Intensive Care, Jena, Germany. tobias.schuerholz@med.uni-jena.de

European Journal of Anaesthesiology
|July 28, 2005
PubMed
Summary

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Plasma levels of terminal complement complex (C5b-9) may not reliably indicate complement activation in septic shock with capillary leak syndrome, despite tissue deposition.

Area of Science:

  • Critical Care Medicine
  • Immunology
  • Nephrology

Background:

  • Sepsis-induced septic shock often involves capillary leak syndrome.
  • Complement activation is implicated in the pathophysiology of septic shock.
  • Terminal complement complex (C5b-9) is a marker of complement system activation.

Purpose of the Study:

  • To evaluate terminal complement complex (C5b-9) plasma levels as a marker for complement activation.
  • To assess the relationship between C5b-9 and capillary leak syndrome in septic shock.

Main Methods:

  • Prospective animal study using pigs with induced fecal peritonitis.
  • Measurement of albumin escape rate to quantify capillary leak.
  • Quantification of plasma C5b-9 levels via immunoassay.

Related Experiment Videos

  • Immunohistological analysis of renal specimens for C5b-9 deposition.

    • Main Results:

    • Septic animals exhibited a significantly increased albumin escape rate (+52%) compared to controls (+3%).
    • Plasma C5b-9 levels decreased over the study period in both septic and control groups.
    • Significant deposition of C5b-9 was observed in renal specimens of septic animals.

    Conclusions:

    • Complement activation was confirmed by renal C5b-9 deposition in septic shock.
    • Decreasing plasma C5b-9 levels during the study suggest it may not be a reliable marker in this context.
    • Plasma C5b-9 levels may not accurately reflect complement activation in septic shock with capillary leak syndrome.