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Updated: Jul 27, 2026

Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein
Published on: March 31, 2010
Substrate binding modulates the reduction potential of DNA photolyase
Yvonne M Gindt1, Johannes P M Schelvis, Katie L Thoren
1Department of Chemistry, Lafayette College, Easton, Pennsylvania 18042, USA. gindty@lafayette.edu
Abstract:
The reduction potential of the (FADH-/FADH*) couple in DNA photolyase was measured, and the value was found to be significantly higher than the values estimated in the literature. In the absence of substrate, the enzyme has a reduction potential of 16 +/- 6 mV vs NHE. In the presence of excess substrate the reduction potential increases to 81 +/- 8 mV vs NHE. The increase in reduction potential has physiological relevance since it gives the catalytic state greater resistance to oxidation. This is the first measurement of a reduction potential for this class of DNA-repair enzymes and the larger family of blue-light photoreceptors.
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