Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Essential role for survivin in early brain development.

Yuying Jiang1, Alain de Bruin, Hugo Caldas

  • 1Center for Childhood Cancer, Columbus Children's Research Institute, Columbus, Ohio 43205, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|July 29, 2005
PubMed
Summary

Survivin is crucial for preventing excessive neuronal cell death during brain development. Deleting survivin in developing neurons causes widespread apoptosis and lethality in newborn mice.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Essential Role for Senescent Cells in Optimal Wound Healing through Secretion of PDGF-AA.

Developmental cell·2026
Same author

Pediatric Brain Tumor Consortium phase 1 study of CD40 agonist sotigalimab in pediatric and young adult patients with recurrent CNS tumors and newly-diagnosed DIPG.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Early penile prosthesis infections in the contemporary antimicrobial era: Incidence and predictive factors from a regional cohort.

BJUI compass·2026
Same author

Effects of Geometrically Centered and Offset Soft Multifocal Optics on Vision Quality in Myopes.

Contact lens & anterior eye : the journal of the British Contact Lens Association·2026
Same author

Non-cell-autonomous mechanisms of tumor initiation and relapse by chromosomal instability.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Tempered Optimism: Advances in the Precision Medicine Era for Pediatric Low-Grade Glioma.

Neuro-oncology·2026

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Apoptosis, or programmed cell death, is vital for normal neuronal development.
  • Significant neuron loss occurs during central nervous system (CNS) maturation.
  • The role of survivin, an inhibitor of apoptosis protein, in neurogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the function of survivin in neuronal precursor cells during embryonic development.
  • To determine the consequences of survivin deficiency on neurogenesis and CNS formation in vivo.

Main Methods:

  • Utilized the Cre-loxP system to generate conditional knockout mice with survivin deleted in neuronal precursor cells.
  • Analyzed developmental apoptosis, survival rates, and CNS morphology in mutant mice.

Related Experiment Videos

  • Assessed caspase and bax expression levels in the brains of conditional mutants.

    • Main Results:

    • Conditional deletion of survivin from embryonic day 10.5 resulted in massive apoptosis of CNS neuronal precursor cells.
    • Mutant mice exhibited reduced brain size and multifocal apoptosis across the CNS and retina.
    • Elevated caspase-3 and caspase-9 activities were observed, while bax expression remained unchanged.

    Conclusions:

    • Survivin is essential for the survival of developing CNS neurons.
    • This finding has implications for understanding neural repair, development, and neurodegenerative diseases.
    • The study establishes survivin's critical antiapoptotic role in vivo during normal neuronal development.