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Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
Study on EDTA-degrading bacterium Burkholderia cepacia YL-6 for bioaugmentation
Shih-Chin Chen1, Szu-Lin Chen, Hung-Yuan Fang
1Graduate Institute of Safety, Health and Environmental Engineering, National Yunlin University of Science and Technology, Touliu, Yunlin 640, Taiwan. csc@ylepb.gov.tw
Abstract:
Bioaugmentation production of EDTA-degrading bacterium Burkholderia cepacia YL-6 was carried out in an aerobic fermentor. Three different carbon sources (ferric-ethylenediaminetetraacetate (Fe-EDTA), potassium acetate, and ethylamine) were used. The bacterium cultivated with Fe-EDTA and maintained in the growth phase could reach the maximum cell concentration on the 38th day. Whereas, the bacterium cultivated with potassium acetate and ethylamine reach the maximum cell concentration at the 76th and 100th hour. The viable-cell counts of the augmentation agents made by feeding Fe-EDTA, potassium acetate, and ethylamine were 8.2x10(10), 6.8x10(11), and 4.3x10(11) CFU/g agent, respectively. The EDTA-degradation time required for the afore-mentioned bioaugmentation agents made by feeding various carbon sources lay in the following order: ethylamine
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