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Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells
Published on: May 24, 2018
Staphylococcal enterotoxin A modulates intracellular Ca2+ signal pathway in human intestinal epithelial cells
Dong-Liang Hu1, Sechiko Suga, Katsuhiko Omoe
1Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan.
Abstract:
We demonstrate here that staphylococcal enterotoxin A (SEA) induces an increase in intracellular calcium ([Ca2+]i) in human intestinal epithelial cells and the [Ca2+]i is released from intracellular stores. SEA-induced increase of [Ca2+]i was clearly inhibited by treatment with a nitric oxide synthase (NOS) inhibitors, N(G)-monomethyl-L-arginine and guanidine. Intestinal epithelial cells express endothelial NOS in resting cell condition, and express inducible NOS after stimulating with tumor necrosis factor (TNF)-alpha. TNF-alpha-pretreated cells showed a significant increase in [Ca2+]i that was also inhibited by the NOS inhibitor. These results suggest that SEA modulated [Ca2+]i signal is dependent on NOS expression in human intestinal epithelial cells.
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