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Updated: Aug 3, 2026
Isolation of Lymphocytes from Mouse Genital Tract Mucosa
Published on: September 3, 2012
Immunization with native surface protein NcSRS2 induces a Th2 immune response and reduces congenital Neospora caninum
G J Haldorson1, B A Mathison, K Wenberg
1Department of Veterinary Microbiology and Pathology, Washington State University, Bustad Hall, Pullman, WA 99164-7040, USA.
Abstract:
NcSRS2, a tachyzoite surface protein of Neospora caninum, is an immunodominant protein with respect to induction of antibody production and has a role in attachment and invasion of host cells. Native NcSRS2 was isolated from whole tachyzoite lysate antigen by affinity chromatography using NcSRS2 specific monoclonal antibody and used to immunize BALB/c mice in a congenital transmission study. NcSRS2 was a highly conserved protein as indicated by comparison of deduced amino acid sequence obtained from NcSRS2 gene sequences of 10 geographically distinct N. caninum isolates. Mice immunized with purified native NcSRS2 produced antigen-specific antibody, primarily of IgG 1 subtype. Following challenge during gestation with 10(7) tachyzoites, immunized mice had a statistically significant decreased frequency of congenital transmission compared to non-immunized mice (P
