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Synthesis of phosphatidylcholine: an improved method without using the cadmium chloride complex of
Ken'ichi Ichihara1, Hitomi Iwasaki, Kaori Ueda
1Biological Chemistry, Graduate School of Agricultural Science, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan. ichihara@kpu.ac.jp
Abstract:
An improved safe method that does not contaminate the environment with cadmium chloride, a toxic heavy metal salt, was developed for the synthesis of phosphatidylcholine (PC). PC was synthesized from sn-glycero-3-phosphocholine (GPC) and fatty acid in one step under mild conditions without the use of cadmium chloride. GPC was prepared from egg yolk PC and adsorbed by kieselguhr in a Teflon vessel. The GPC on kieselguhr was acylated with fatty acid in the presence of two reagents, dicyclohexylcarbodiimide for synthesis of fatty acid anhydride and 4-dimethylaminopyridine as an acylating catalyst, at 30 degrees C overnight. The PC thus produced was purified by silica gel column chromatography. The yield of dioleoyl PC was 90% based on the starting material, GPC.
