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Updated: Aug 13, 2026
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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Published on: June 5, 2015
Recombinant globulins: novel research tools and possible pharmaceuticals
D Hollenbaugh1, N J Chalupny, A Aruffo
1Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington.
Current Opinion in Immunology
|April 1, 1992
Summary
Researchers created novel protein chimeras by fusing target proteins with immunoglobulin constant domains. These recombinant globulins aid in identifying ligand-receptor pairs and understanding protein functions.
Area of Science:
- Protein engineering
- Immunology
- Biochemistry
Background:
- Studying protein interactions is crucial for understanding biological processes.
- Existing methods for protein analysis have limitations in certain applications.
Purpose of the Study:
- To introduce a novel method for protein study using engineered protein chimeras.
- To highlight the utility of these chimeras in various biological and therapeutic applications.
Main Methods:
- Construction of chimeras by fusing target proteins to immunoglobulin constant domains.
- Utilizing these recombinant globulins for protein interaction studies.
Main Results:
- Successful creation of functional protein chimeras.
- Demonstrated efficacy in identifying ligand-receptor pairs.
- Enabled determination of functional consequences of receptor engagement.
- Aided in elucidating structural domains critical for ligand binding.
Conclusions:
- Protein chimeras offer a versatile approach for protein research.
- These recombinant molecules have broad applications, including potential therapeutic uses.
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