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Single cell protein production from beet pulp by mixed culture
1Botany Department, Faculty of Science, Alex. Univ., Egypt.
Summary
Mixed cultures of Trichoderma reesei and Kluyveromyces marxianus efficiently convert beet pulp into high-yield single-cell proteins (SCP). Optimized conditions increased protein yield to 54% and conversion efficiency to 41.8%.
Area of Science:
- Microbiology
- Biotechnology
- Food Science
Background:
- Beet pulp (BP) is an abundant lignocellulosic byproduct with potential for bioconversion.
- Single-cell protein (SCP) production using microbial fermentation offers a sustainable protein source.
Purpose of the Study:
- To evaluate the efficacy of mixed microbial cultures for enhanced SCP production from BP.
- To optimize fermentation conditions for maximizing protein yield and BP conversion efficiency.
Main Methods:
- Cultivation of mixed cultures of Trichoderma reesei and Kluyveromyces marxianus on BP-containing media.
- Optimization of BP concentration and yeast extract substitution in the basal medium.
- Analysis of single-cell protein (SCP) yield and BP conversion efficiency.
Main Results:
- The mixed culture of T. reesei and K. marxianus achieved high SCP yields (51%) and efficient BP conversion (39.4%).
- Substituting yeast extract and increasing BP concentration to 4% significantly enhanced performance.
- Optimized conditions resulted in maximum protein yields of 54% and BP conversion efficiency of 41.8%.
Conclusions:
- Mixed microbial fermentation, particularly with T. reesei and K. marxianus, is a viable strategy for high-value SCP production from beet pulp.
- The resulting protein contains all essential amino acids, comparable to established protein sources.
- This approach offers a sustainable and efficient method for valorizing agricultural byproducts into protein-rich biomass.