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Related Experiment Videos

Ab initio phase determination for spherical viruses: parameter determination for spherical-shell models.

M S Chapman1, J Tsao, M G Rossmann

  • 1Purdue University, Department of Biological Sciences, West Lafayette, Indiana 47907.

Acta Crystallographica. Section A, Foundations of Crystallography
|May 1, 1992
PubMed
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This study demonstrates ab initio phasing for canine parvovirus structure determination. Precise refinement of parameters from diffraction data enables phase extension from 20 Å resolution, potentially speeding up spherical virus capsid analysis.

Area of Science:

  • Structural biology
  • Virology
  • Crystallography

Background:

  • Canine parvovirus structure determination previously relied on experimental phasing methods.
  • Ab initio phasing offers a promising alternative to expedite structural analysis of spherical viruses.

Purpose of the Study:

  • To demonstrate the feasibility of ab initio phasing for canine parvovirus.
  • To refine parameters for precise structure determination without initial experimental phasing.

Main Methods:

  • Utilizing a spherical-shell model for initial phase calculation.
  • Refining parameters like radii, DNA density, and particle positions from diffraction data.
  • Extending phases from 20 Å resolution.

Main Results:

Related Experiment Videos

  • Successful determination and refinement of key structural parameters.
  • Demonstrated precise determination of radii, DNA density, and particle positions.
  • Achieved phase extension from 20 Å resolution for a virus of ~122 Å radius.

Conclusions:

  • Ab initio phasing is a viable method for canine parvovirus structure determination.
  • This approach can reduce the time and resources required for structural analysis.
  • Expedited structure determination of spherical virus capsids is achievable.