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Updated: Aug 16, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
[HPLC determination of metformin hydrochloride-related substances]
Lin-run Wang1, Ming-zhu Huang, Su-hui Zhu
1The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. Linrunw@Yahoo.com.cn
Objective:
To develop a HPLC assay for the determination of metformin hydrochloride-related substances.
Methods:
The separation was performed on SHIMADZU VP-ODS (250 4.6 mm, 5 microm) column. The mobile phase of dicyandiamide was composed of methyl alcohol-1 mmol x L(-1) sodium dodecylsulfate in 10 mmol x L(-1) phosphate salt solution (60:40) (pH=5.5). The mobile phase of other related substances was composed of methyl alcohol-1 mmol x L(-1) sodium dodecysulfate in 10 mmol x L(-1) phosphate salt solution (55:45)(pH=5.5). The detection wavelength was 232 nm, and the running speed was 0.8 ml min(-1) at room temperature.
Result:
Good resolution of dicyandiamide and main peak was obtained. The test results were reproducible.
Conclusion:
The method is simple, rapid and suitable for the determination of dicyandiamide and other metformin hydrochloride-related substances.
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