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Directional enrichment of directly cloned PCR products

David Horn1

  • 1London School of Hygiene & Tropical Medicine, London, UK. david.horn@lshtm.ac.uk

Biotechniques
|August 3, 2005
PubMed
Abstract

No abstract available in PubMed .

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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