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Related Experiment Videos

Fluorescent sensor for Cu2+ with a tunable emission wavelength.

Andriy Mokhir1, Alexander Kiel, Dirk-Peter Herten

  • 1Institute of Inorganic Chemistry, Ruprecht-Karls-Universität Heidelberg, Germany. Andriy.Mokhir@urz.uni-heidelberg.de

Inorganic Chemistry
|August 3, 2005
PubMed
Summary

This study presents a novel fluorescent sensor for detecting copper ions (Cu2+). The sensor utilizes tunable DNA-based probes and fluorescence resonance energy transfer (FRET) for sensitive and selective metal ion detection.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Fluorescent metal ion sensors are crucial for environmental and biological monitoring.
  • Existing sensors often lack tunable emission wavelengths, limiting their application in complex biological samples with autofluorescence.
  • Developing sensors with adjustable emission spectra is essential for improved sensitivity and specificity.

Purpose of the Study:

  • To develop a fluorescent metal ion sensor with an easily tunable emission wavelength.
  • To demonstrate the sensor's principle through the detection of copper ions (Cu2+).
  • To achieve sensitive detection of Cu2+ with a low limit of detection.

Main Methods:

  • Chemically modifying fluorescein dye with a metal chelating group and attaching it to ss-DNA.

Related Experiment Videos

  • Combining the modified fluorescein-ss-DNA with a complementary ATTO 590-modified ss-DNA to form a duplex.
  • Utilizing fluorescence resonance energy transfer (FRET) between the donor and acceptor dyes within the DNA duplex.
  • Monitoring the quenching of ATTO 590 emission upon coordination of Cu2+ ions by the chelating unit.

    • Main Results:

    • Successfully demonstrated FRET between fluorescein and ATTO 590 dyes within a DNA duplex.
    • Showed that Cu2+ coordination quenches both fluorescein and ATTO 590 emissions.
    • Achieved a detection limit of 20 nM for Cu2+ using the ATTO 590 emission.
    • Demonstrated the tunability of the emission wavelength by changing the acceptor DNA-dye conjugate.

    Conclusions:

    • The developed sensor provides a sensitive and selective method for Cu2+ detection.
    • The tunable emission wavelength offers significant advantages for applications in biological samples by avoiding autofluorescence.
    • This platform enables the development of a new class of rare fluorescent sensors emitting above 600 nm.