Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Activating AMP-activated protein kinase without AMP.

Morris J Birnbaum1

  • 1Howard Hughes Medical Institute and The Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.

Molecular Cell
|August 3, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A newly revealed signaling pathway may explain the conundrum of how FGF21 simultaneously increases insulin sensitivity and autophagy.

Journal of hepatology·2025
Same author

SPAG7 deletion causes intrauterine growth restriction, resulting in adulthood obesity and metabolic dysfunction.

eLife·2024
Same author

Hepatic Activin E mediates liver-adipose inter-organ communication, suppressing adipose lipolysis in response to elevated serum fatty acids.

Molecular metabolism·2024
Same author

Regulated dynamic subcellular GLUT4 localization revealed by proximal proteome mapping in human muscle cells.

Journal of cell science·2023
Same author

Chronic UCN2 treatment desensitizes CRHR2 and improves insulin sensitivity.

Nature communications·2023
Same author

GLUT4 dynamic subcellular localization is controlled by AMP kinase activation as revealed by proximal proteome mapping in human muscle cells.

bioRxiv : the preprint server for biology·2023
Same journal

Thyroid cancer-associated EZH1 Q571R mutation drives chromatin compaction and H3K27me3 invasion into active chromatin.

Molecular cell·2026
Same journal

Genome-wide rotational and translational phasing of nucleosomes with human transcription factors.

Molecular cell·2026
Same journal

Spliceosomal proofreading factors safeguard 3' splice-site fidelity and prevent proteotoxicity and inflammation.

Molecular cell·2026
Same journal

Cytosolic EZH2-IMPDH2 complexes regulate melanoma progression and metastasis via GTP.

Molecular cell·2026
Same journal

A bacterial reverse transcriptase: Protein-templated DNA synthesis fuels antiviral immunity.

Molecular cell·2026
Same journal

Tweezing apart ribosome heterogeneity.

Molecular cell·2026
See all related articles

AMPK activation was previously thought to depend solely on cellular energy levels. New research reveals a calcium-dependent signaling pathway also activates AMPK, expanding our understanding of its regulation.

Area of Science:

  • Biochemistry
  • Cellular signaling

Background:

  • Adenosine monophosphate-activated protein kinase (AMPK) is a crucial cellular energy sensor.
  • AMPK is traditionally understood to be regulated by the cellular energy state, primarily through changes in AMP/ATP ratios.

Purpose of the Study:

  • To investigate novel regulatory mechanisms of AMPK beyond cellular energy status.
  • To explore the role of calcium signaling in AMPK activation.

Main Methods:

  • Utilized cell-based assays to monitor AMPK activity.
  • Employed calcium imaging techniques to correlate calcium levels with AMPK phosphorylation.
  • Investigated the effects of calcium-modulating agents on AMPK activation.

Main Results:

Related Experiment Videos

  • Demonstrated that AMPK can be activated independently of cellular energy levels.
  • Identified a direct link between calcium influx and AMPK activation.
  • Showcased that specific calcium-dependent signaling pathways trigger AMPK phosphorylation.

    • Conclusions:

    • AMPK regulation is more complex than previously assumed, involving both energy-dependent and calcium-dependent pathways.
    • Calcium signaling represents a significant, previously underappreciated, pathway for AMPK activation.
    • These findings open new avenues for therapeutic strategies targeting AMPK in various physiological and pathological conditions.