Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Field-flow fractionation and biotechnology.

Pierluigi Reschiglian1, Andrea Zattoni, Barbara Roda

  • 1Department of Chemistry G. Ciamician, University of Bologna, Via Selmi 2, I-40126 Bologna, Italy.

Trends in Biotechnology
|August 3, 2005
PubMed
Summary

Field-flow fractionation (FFF) offers gentle sample separation for biotechnology, including proteins, cells, and viruses. Advances in FFF technology and coupled techniques are expanding its applications in areas like vaccine production and proteomics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Color-Tuning Bioluminescent Sensor (AmyLuc) for Real-Time Monitoring of Intracellular pH Dynamics in Cancer Cells.

Analytical chemistry·2026
Same author

Novel Smartphone Paper Sensor for One Health: Monitoring Free Chlorine in Water and Exhaled Breath Condensate.

Sensors (Basel, Switzerland)·2026
Same author

Online Coupling of Field-Flow Fractionation with Raman Microspectroscopy Enables the Advanced Study of Nanoplastics Directly in Food.

Analytical chemistry·2025
Same author

FFF as a microfluidic platform for the streamlined optimization of ready-to-use nanozyme-labelled probes to enable robust and ultra-sensitive chemiluminescent bioassays.

Biosensors & bioelectronics·2025
Same author

Hollow fiber flow field-flow fractionation enables high-quality Extracellular Vesicle isolation from minimal plasma samples in polycythemia vera liquid biopsy.

Talanta·2025
Same author

Jagged1-Fluc: A Luminescent Recombinant Protein as a Sensitive Tool for High-Throughput Screening of Colorectal Cancer.

BioFactors (Oxford, England)·2025

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biophysics

Background:

  • Field-flow fractionation (FFF) is a versatile separation technique.
  • Its gentle mechanism is ideal for delicate biological samples like proteins, nucleic acids, viruses, and cells.
  • Recent technological advancements are broadening its scope.

Purpose of the Study:

  • To highlight recent progress in field-flow fractionation (FFF) technology.
  • To explore emerging biotechnological applications driven by FFF advancements and coupled techniques.
  • To showcase the potential of FFF in diverse fields from cell sorting to proteomics.

Main Methods:

  • Field-flow fractionation (FFF) for sample separation.
  • Coupling FFF with established analytical methods (e.g., mass spectrometry, chromatography).

Related Experiment Videos

  • Application-specific method development for cell sorting, proteomics, and immunoassays.

    • Main Results:

    • Demonstrated successful sorting and fingerprinting of bacteria for vaccine production.
    • Enabled noninvasive, tagless sorting of immature and stem cells.
    • Facilitated separation of intact proteins and enzymes for top-down proteomics.
    • Developed flow-assisted immunoassays using functionalized nano- and micron-sized particles.

    Conclusions:

    • Field-flow fractionation (FFF) is a powerful and adaptable technology for complex biological samples.
    • Advancements in FFF and hyphenated techniques are unlocking novel applications in biotechnology.
    • FFF shows significant promise for applications in diagnostics, therapeutics, and fundamental biological research.