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Mass isotopomer pattern and precursor-product relationship.

W N Lee1, E A Bergner, Z K Guo

  • 1Department of Pediatrics, Harbor-UCLA Medical Center, Torrance 90502.

Biological Mass Spectrometry
|February 1, 1992
PubMed
Summary
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Determining polymer precursor enrichment is possible using mass isotopomer distribution analysis. This method accurately quanties 13C enrichment in polymers like glucose pentaacetate, unaffected by product dilution.

Area of Science:

  • Chemical Synthesis
  • Analytical Chemistry
  • Polymer Science

Background:

  • Homocleus polymers synthesized from labeled precursors exhibit mass isotopomer distributions.
  • This distribution is directly related to the enrichment of the 13C-labeled precursor.

Purpose of the Study:

  • To investigate the mass isotopomer pattern of glucose pentaacetate synthesized from 13C-enriched acetic anhydride.
  • To demonstrate the utility of isotopomer patterns for determining precursor enrichment and product dilution in polymer synthesis.

Main Methods:

  • Synthesis of glucose pentaacetate from 13C-enriched acetic anhydride.
  • Mass isotopomer distribution analysis of the tetraacetyl moiety (m/z 331).
  • Correction for natural abundance of 13C.
  • Plotting mass isotopomer ratios against observed enrichment.

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Main Results:

  • A linear relationship was observed between the mass isotopomer ratio (m4/m2) and the enrichment of the tetraacetate moiety.
  • The observed slope (1.45) agreed with the theoretical formula for consecutive mass isotopomers.
  • Product dilution with unenriched material did not alter the isotopomer ratio.

Conclusions:

  • Mass isotopomer pattern analysis is a reliable method for determining precursor enrichment in polymer synthesis.
  • The method is robust and unaffected by the presence of unlabeled product.
  • This technique offers a practical approach for quantifying enrichment in de novo polymer synthesis.